Brighton U21 will join the Bees, Cambridge United and Luton Town in Group H of the 2025/26 Vertu Trophy.
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The top two from each group progress to the knockout stages, with Vertu Trophy fixtures remaining regionalised until the Quarter-Final stage.
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Matches level after ninety minutes will be determined by penalty kicks up to the Semi-Final stage.
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A period of 30 minutes of extra time will be played in the Final should the match be level after ninety minutes. If still level at the end of extra time, the winners shall be determined by penalty kicks.
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The EFL’s record domestic rights deal with broadcaster Sky Sports will see every Vertu Trophy match broadcast live on Sky Sports for fans in the UK.